Oxidative stress is described as cell damage caused by free radicals, or unstable molecules, which can ultimately affect healthy function. The human body creates free radicals to neutralize bacteria and viruses, however, external factors, such as oxygen, pollution, and radiation, can often also produce free radicals. Oxidative stress has been associated with numerous health issues.
Oxidative stress and other stressors turn on internal protective mechanisms which can help regulate the human body’s antioxidant response. Nrf2 is a protein which senses levels of oxidative stress and enables the cells to protect themselves from internal and external factors. Nrf2 has also been demonstrated to help regulate genes involved in the production of antioxidant enzymes and stress-response genes. The purpose of the article below is to explain the effects of Nrf2 in cancer.
The Keap1-Nrf2 pathway is the major regulator of cytoprotective responses to oxidative and electrophilic stress. Although cell signaling pathways triggered by the transcription factor Nrf2 prevent cancer initiation and progression in normal and premalignant tissues, in fully malignant cells Nrf2 activity provides growth advantage by increasing cancer chemoresistance and enhancing tumor cell growth. In this graphical review, we provide an overview of the Keap1-Nrf2 pathway and its dysregulation in cancer cells. We also briefly summarize the consequences of constitutive Nrf2 activation in cancer cells and how this can be exploited in cancer gene therapy.
The Keap1-Nrf2 pathway is the major regulator of cytoprotective responses to endogenous and exogenous stresses caused by reactive oxygen species (ROS) and electrophiles . The key signaling proteins within the pathway are the transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2) that binds together with small Maf proteins to the antioxidant response element (ARE) in the regulatory regions of target genes, and Keap1 (Kelch ECH associating protein 1), a repressor protein that binds to Nrf2 and promotes its degradation by the ubiquitin proteasome pathway (Fig. 1). Keap1 is a very cysteine-rich protein, mouse Keap1 having a total of 25 and human 27 cysteine residues, most of which can be modified in vitro by different oxidants and electrophiles . Three of these residues, C151, C273 and C288, have been shown to play a functional role by altering the conformation of Keap1 leading to nuclear translocation of Nrf2 and subsequent target gene expression  (Fig. 1). The exact mechanism whereby cysteine modifications in Keap1 lead to Nrf2 activation is not known, but the two prevailing but not mutually exclusive models are (1) the �hinge and latch� model, in which Keap1 modifications in thiol residues residing in the IVR of Keap1 disrupt the interaction with Nrf2 causing a misalignment of the lysine residues within Nrf2 that can no longer be polyubiquitinylated and (2) the model in which thiol modification causes dissociation of Cul3 from Keap1 . In both models, the inducer-modified and Nrf2-bound Keap1 is inactivated and, consequently, newly synthesized Nrf2 proteins bypass Keap1 and translocate into the nucleus, bind to the ARE and drive the expression of Nrf2 target genes such as NAD(P)H quinone oxidoreductase 1 (NQO1), heme oxygenase 1 (HMOX1), glutamate-cysteine ligase (GCL) and glutathione S transferases (GSTs) (Fig. 2). In addition to modifications of Keap1 thiols resulting in Nrf2 target gene induction, proteins such as p21 and p62 can bind to Nrf2 or Keap1 thereby disrupting the interaction between Nrf2 and Keap1 ,  (Fig. 3).
Mechanisms of Activation and Dysregulation of Nrf2 in Cancer
Although cytoprotection provided by Nrf2 activation is important for cancer chemoprevention in normal and premalignant tissues, in fully malignant cells Nrf2 activity provides growth advantage by increasing cancer chemoresistance and enhancing tumor cell growth . Several mechanisms by which Nrf2 signaling pathway is constitutively activated in various cancers have been described: (1) somatic mutations in Keap1 or the Keap1 binding domain of Nrf2 disrupting their interaction; (2) epigenetic silencing of Keap1 expression leading to defective repression of Nrf2; (3) accumulation of disruptor proteins such as p62 leading to dissociation of the Keap1-Nrf2 complex; (4) transcriptional induction of Nrf2 by oncogenic K-Ras, B-Raf and c-Myc; and (5) post-translational modification of Keap1 cysteines by succinylation that occurs in familial papillary renal carcinoma due to the loss of fumarate hydratase enzyme activity , , , , , , ,  (Fig. 3). Constitutively abundant Nrf2 protein causes increased expression of genes involved in drug metabolism thereby increasing the resistance to chemotherapeutic drugs and radiotherapy. In addition, high Nrf2 protein level is associated with poor prognosis in cancer . Overactive Nrf2 also affects cell proliferation by directing glucose and glutamine towards anabolic pathways augmenting purine synthesis and influencing the pentose phosphate pathway to promote cell proliferation  (Fig. 4).
Given that high Nrf2 activity commonly occurs in cancer cells with adverse outcomes, there is a need for therapies to inhibit Nrf2. Unfortunately, due to structural similarity with some other bZip family members, the development of specific Nrf2 inhibitors is a challenging task and only a few studies of Nrf2 inhibition have been published to date. By screening natural products, Ren et al.  identified an antineoplastic compound brusatol as an Nrf2 inhibitor that enhances the chemotherapeutic efficacy of cisplatin. In addition, PI3K inhibitors ,  and Nrf2 siRNA  have been used to inhibit Nrf2 in cancer cells. Recently, we have utilized an alternative approach, known as cancer suicide gene therapy, to target cancer cells with high Nrf2 levels. Nrf2-driven lentiviral vectors  containing thymidine kinase (TK) are transferred into cancer cells with high ARE activity and the cells are treated with a pro-drug, ganciclovir (GCV). GCV is metabolized to GCV-monophosphate, which is further phosphorylated by cellular kinases into a toxic triphosphate form  (Fig. 5). This leads to effective killing of not only TK containing tumor cells, but also the neighboring cells due to the bystander effect . ARE-regulated TK/GCV gene therapy can be further enhanced via combining a cancer chemotherapeutic agent doxorubicin to the treatment , supporting the notion that this approach could be useful in conjuction with traditional therapies.
Nrf2 is a master regulator which triggers the production of powerful antioxidants in the human body which help eliminate oxidative stress. Various antioxidant enzymes, such as superoxide dismutase, or SOD, glutathione, and catalase, are also activated through the Nrf2 pathway. Furthermore, certain phytochemicals like turmeric, ashwagandha, bacopa, green tea, and milk thistle, activate Nrf2. Research studies have found that Nrf2 activation can naturally enhance cellular protection and restore balance to the human body.
Dr. Alex Jimenez D.C., C.C.S.T. Insight
Sulforaphane and Its Effects on Cancer, Mortality, Aging, Brain and Behavior, Heart Disease & More
Isothiocyanates are some of the most important plant compounds you can get in your diet. In this video I make the most comprehensive case for them that has ever been made. Short attention span? Skip to your favorite topic by clicking one of the time points below. Full timeline below.
00:01:14 – Cancer and mortality
00:19:04 – Aging
00:26:30 – Brain and behavior
00:38:06 – Final recap
00:40:27 – Dose
00:00:34 – Introduction of sulforaphane, a major focus of the video.
00:01:14 – Cruciferous vegetable consumption and reductions in all-cause mortality.
00:02:12 – Prostate cancer risk.
00:02:23 – Bladder cancer risk.
00:02:34 – Lung cancer in smokers risk.
00:02:48 – Breast cancer risk.
00:03:13 – Hypothetical: what if you already have cancer? (interventional)
00:03:35 – Plausible mechanism driving the cancer and mortality associative data.
00:04:38 – Sulforaphane and cancer.
00:05:32 – Animal evidence showing strong effect of broccoli sprout extract on bladder tumor development in rats.
00:06:06 – Effect of direct supplementation of sulforaphane in prostate cancer patients.
00:07:09 – Bioaccumulation of isothiocyanate metabolites in actual breast tissue.
00:08:32 – Inhibition of breast cancer stem cells.
00:08:53 – History lesson: brassicas were established as having health properties even in ancient Rome.
00:09:16 – Sulforaphane’s ability to enhance carcinogen excretion (benzene, acrolein).
00:09:51 – NRF2 as a genetic switch via antioxidant response elements.
00:10:10 – How NRF2 activation enhances carcinogen excretion via glutathione-S-conjugates.
00:10:34 – Brussels sprouts increase glutathione-S-transferase and reduce DNA damage.
00:11:20 – Broccoli sprout drink increases benzene excretion by 61%.
00:13:31 – Broccoli sprout homogenate increases antioxidant enzymes in the upper airway.
00:15:45 – Cruciferous vegetable consumption and heart disease mortality.
00:16:55 – Broccoli sprout powder improves blood lipids and overall heart disease risk in type 2 diabetics.
00:19:04 – Beginning of aging section.
00:19:21 – Sulforaphane-enriched diet enhances lifespan of beetles from 15 to 30% (in certain conditions).
00:20:34 – Importance of low inflammation for longevity.
00:22:05 – Cruciferous vegetables and broccoli sprout powder seem to reduce a wide variety of inflammatory markers in humans.
00:36:32 – Sulforaphane improves learning in model of type II diabetes in mice.
00:37:19 – Sulforaphane and duchenne muscular dystrophy.
00:37:44 – Myostatin inhibition in muscle satellite cells (in vitro).
00:38:06 – Late-video recap: mortality and cancer, DNA damage, oxidative stress and inflammation, benzene excretion, cardiovascular disease, type II diabetes, effects on the brain (depression, autism, schizophrenia, neurodegeneration), NRF2 pathway.
00:40:27 – Thoughts on figuring out a dose of broccoli sprouts or sulforaphane.
00:41:01 – Anecdotes on sprouting at home.
00:43:14 – On cooking temperatures and sulforaphane activity.
00:43:45 – Gut bacteria conversion of sulforaphane from glucoraphanin.
00:44:24 – Supplements work better when combined with active myrosinase from vegetables.
00:44:56 – Cooking techniques and cruciferous vegetables.
00:46:06 – Isothiocyanates as goitrogens.
This work was supported by the Academy of Finland, the Sigrid Juselius Foundation and the Finnish Cancer Organisations.
In conclusion, nuclear factor (erythroid-derived 2)-like 2, also known as NFE2L2 or Nrf2, is a protein which increases the production of antioxidants which protect the human body against oxidative stress. As described above, the stimulation of the Nrf2 pathway are being studies for the treatment of diseases caused by oxidative stress, including cancer. The scope of our information is limited to chiropractic and spinal health issues. To discuss the subject matter, please feel free to ask Dr. Jimenez or contact us at�915-850-0900�.
Additional Topic Discussion: Relieving Knee Pain without Surgery
Knee pain is a well-known symptom which can occur due to a variety of knee injuries and/or conditions, including�sports injuries. The knee is one of the most complex joints in the human body as it is made-up of the intersection of four bones, four ligaments, various tendons, two menisci, and cartilage. According to the American Academy of Family Physicians, the most common causes of knee pain include patellar subluxation, patellar tendinitis or jumper’s knee, and Osgood-Schlatter disease. Although knee pain is most likely to occur in people over 60 years old, knee pain can also occur in children and adolescents. Knee pain can be treated at home following the RICE methods, however, severe knee injuries may require immediate medical attention, including chiropractic care.
Hypothyroidism can be a tricky condition to handle, and what you eat could interfere with your treatment. Some nutrients influence the function of the thyroid gland, and certain foods can inhibit your body’s ability to absorb them.
What foods can affect thyroid disease?
Having a thyroid condition is often difficult, but you are not alone with this particular health issue. According to the American Thyroid Association, more than 12 percent of the populace may wind up coping with a thyroid disease.
As with many health conditions, some factors are out of your control, such as your family history and the environment around you. But nutrition and diet also plays a role in thyroid health and since you’re the one in control of your plate, then you can decide which thyroid-friendly foods to pick as you handle hypothyroidism and its symptoms.
Foods with Soy (Edamame, Tofu, and Miso)
There’s long been concern over the potential negative effects that certain compounds in soy, called isoflavones, may have on the thyroid gland. Some researchers think that a person’s risk for hypothyroidism can increase. However others theorize that those with both hypothyroidism and an iodine deficiency should observe their intake.
So there are no specific nutritional guidelines regarding the consumption of soy, but some studies do indicate that the ingestion of soy may interfere with the ability to intake thyroid drugs and medications. Because of this, you may want to wait four hours before taking your dose, after eating these foods. Check with your doctor.
Cruciferous Vegetables (Broccoli and Cauliflower)
Cruciferous vegetables, such as broccoli and cabbage, are full of fiber and other nutrients, but they could interfere with the production of thyroid gland when you experience an iodine deficiency. Therefore, in case you do, it is a great idea to restrict your intake of Brussels sprouts, cabbage, cauliflower, kale, turnips, and bok choy, since research indicates digesting these veggies may block the thyroid’s ability to utilize iodine, which is vital for normal thyroid function.
If you have been diagnosed with both hypothyroidism and iodine deficiency, there are a number of things you can do to make these vegetables less dangerous. Cooking them can reduce the impact that cruciferous vegetables have on the thyroid gland, and limiting your intake of these (cooked) vegetables to 5 ounces a day can help too, because that amount appears to have no negative impact on thyroid functioning.
Gluten (Bread, Pasta, and Rice)
People who have migraines might wish to look at decreasing their intake of gluten, a protein found in foods processed from barley, wheat, rye, and other grains, ” says Ruth Frechman, RDN, a dietitian in the Los Angeles area and a spokesperson for the Academy of Nutrition and Dietetics. And in case you’re diagnosed with celiac disease, gluten may hamper absorption of thyroid hormone replacement medication, and can irritate the small intestine.
An article published in May 2017 in the journal “Endocrine Connections” noted that celiac and rheumatoid disease tend to be present together, and while no research has demonstrated that a gluten-free diet can treat thyroid problems, you might want to speak to a healthcare professional about whether it might be well worth eliminating gluten, or becoming tested for celiac disease. If you do decide to eat gluten, make sure to choose whole-grains varieties of bread, pasta, and rice, that are high in fiber and other nutrients and can help improve bowel irregularity, a symptom of hypothyroidism.
Fatty Foods (Butter, Meat, and Fried Foods)
Fats have been found to disrupt the human body’s ability to absorb thyroid hormone replacement medicines, says Stephanie Lee, MD, PhD, associate chief of endocrinology, nutrition, and diabetes in Boston Medical Center and an associate professor in the Boston University School of Medicine in Massachusetts.
Fats may also interfere with the thyroid’s ability to produce hormone as well. Some healthcare professionals recommend that you just cut out on foods that are fried and lower your intake of fats from resources such as butter, mayonnaise, margarine, and fatty cuts of beef.
Sugary Foods (Chocolate and Desserts)
Hypothyroidism may cause the body’s metabolism to slow down, Frechman states. That means it’s simple to put on pounds if you aren’t careful. “You would like to avoid foods with excess amounts of sugar because it’s a lot of calories without the nourishment,” she states. Attempt to eliminate it completely or it is best to decrease.
Processed Foods in Packages
“Processed foods generally get lots of sodium, and individuals with hypothyroidism should avoid sodium,” Frechman states. Having an underactive thyroid increases a individual’s risk for high blood pressure, and sodium that is an excessive amount of increases this risk.
Read the “Nutrition Facts” label on the packaging of processed foods to seek out options lowest in sodium. Individuals with an increased risk for hypertension should restrict their sodium intake according to the American Heart Association.
Excessive Fiber (Beans, Legumes, and Vegetables)
Getting enough fiber is good for you, but also much may complicate your hypothyroidism therapy. The government Strategies for Americans recommends that adults choose in 20 to 35 g of fiber a day. Amounts of fiber from fruits, vegetables, whole grains, beans, and legumes which go above that amount affect your digestive tract and may interfere with absorption of thyroid hormone replacement drugs.
If you’re on a high-fiber diet, ask your physician if you will need a higher dose of thyroid medicine. If you aren’t absorbing enough medication your maintenance dose may have to be increased.
Coffee (Time your First Cup Carefully)
Caffeine has been shown to block absorption of thyroid hormone replacement, says Dr. Lee. “People who had been taking their thyroid medication with their morning coffee had uncontrollable thyroid levels, and we couldn’t figure it out,” she states. “I now must be very careful to tell people, ‘Simply take your medicine with water.'” You should wait at least 30 minutes before having a cup of coffee after taking your medication.
Alcohol and Thyroid Health
Alcohol consumption can cause a mess on both thyroid hormone levels in the body and the ability of the thyroid gland to produce these hormones. Alcohol appears to have a toxic effect in the thyroid gland and it also suppresses the ability of the body to utilize thyroid gland hormones. Ideally, individuals with migraines should cut out alcohol completely..
The scope of our information is limited to chiropractic and spinal injuries and conditions. To discuss options on the subject matter, please feel free to ask Dr. Jimenez or contact us at 915-850-0900 .
By Dr. Alex Jimenez
Additional Topics: Wellness
Overall health and wellness are essential towards maintaining the proper mental and physical balance in the body. From eating a balanced nutrition as well as exercising and participating in physical activities, to sleeping a healthy amount of time on a regular basis, following the best health and wellness tips can ultimately help maintain overall well-being. Eating plenty of fruits and vegetables can go a long way towards helping people become healthy.
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